Characterization of Extended Spectrum Beta Lactamase Producing Escherichia coli from Calves

Background: Cephalosporins are major antimicrobials used to treat serious infections. However, their effectiveness is being compromised by the emergence of extended spectrum beta lactamases (ESBL). The present study was aimed characterize ESBL producing Escherichia coli from healthy and diarrheic cattle calves. Methods: A total 52 rectal swabs were collected calves between August 2019 March 2020. Phenotypically E. characterized based on morphology, microscopy biochemical tests. Extended Spectrum Beta Lactamases (ESBL) initial screening performed using cefpodoxime, ceftazidime, cefepime aztreonam antibiotics. confirmation strains conducted through phenotypic identification kit double disc diffusion test (DDDT). Molecular characterization PCR amplification blaCTX-M, bla TEM AmpC genes. Plasmid profiling isolates carried out. Result: Phenotypically, out 51 isolated, 05 (9.8%) confirmed as strains. revealed 01 (20%), 04 (80%) 03 (60%) positive for genes, respectively. showed that 60% harboring plasmids varying size number. plasmid number among ranged 0-4 per isolate varied 900bp greater than 10kb. relatively high rates producers in found highlights existence with certain carrying mobile genetic elements livestock probable disseminators resistance, thus imposing public health threat.